KPL 'Bactrace' FITC Set 2
Some fresh fingertip blood was mixed with KPL product: 02-97-91. KPL state:
"Affinity purified polyclonal antibody to Borrelia burgdorferi made in Goat and labeled with fluorescein isothiocyanate (FITC). Isolated from a serum pool of goats immunized with heat killed whole cells of Borrelia burgdorferi. The antibody is highly specific for Borrelia burgdorferi." (Link to product page)
Method: One drop of fluorescent antibody stain (1:20 distilled water) on centre of slide. Added small drop of fingertip blood and mixed. 50mm coverslip with grease on each corner placed and pushed down until whole area covered with sample. Long sides of coverslip sealed with adhesive. Stored in light-proof container with small vial of water to reduce dessication for 30 hours.
Matched pairs of darkfield and fluorescence filtered images edited only for noise and size. Fluorescence filtered images are the green tinted ones.
Last update January 31st 2014