Borrelia Spirochete Morphology in Peripheral Blood part 2

Results of Experiment Kit 1
Only 1 set of slides were a complete failure and the donor kindly provided a repeat set which was suitable. All the other sets had at least one slide that was useable. With this kit, problems were mainly found to be related to the amount of blood put on the slide. This is described in the discussion below.

Spirochetal forms were usually observed quite easily with the majority producing results between 2 and 4 days after the sample draw. Figure 1 shows images taken at the same time as the video recording, or excerpted from the video itself. The legend with each picture refers to its corresponding video listed below. 1, 2 and 8 are from the same donor. Particularly recommended are videos 3, 8 (note the waveform spirochete at 10 seconds) and 10.

Figure 1. Results of Kit 1. Showing photographs made at the same time that video was recorded or a still image extracted from the video. The legend beneath each panel corresponds to a video file.

Right Click on a number to view the video hosted on Youtube:

1 2 3 4 5 6 7 8 9 Play All Videos

Results of Experiment Kit 2

One set of slides showed rapid decomposition of the erythrocytes though one slide from this set was less affected and this was used. Other sets sometimes contained decaying erythrocytes, usually close to the exposed short edge of the coverslip. One set produced results in just 24 hours though most results occurred between 2 and 4 days as with Kit 1.

Some donors had problems achieving a suitable ratio between BSK and blood. A few had problems handling the adhesive to seal the long edges of the coverslip and the glue sometimes trailed across the coverslip (which was easily removed). Some samples were found to be rather deep which made microscopy awkward. However, every set had at least one slide that was useable. Figure 2 shows photographs from Kit 2. In general the results were better than Kit 1. Videos 15 and 16 are from the same donor. Particularly recommended videos are numbers 10, 13, 14, 15, 16 and 20, the latter concluding with a still image showing a spirochete forming 30 individual coccoids within its membrane, like a 'string of pearls'.

Figure 2. Results of Kit 2. Showing photographs made at the same time that video was recorded or a still image from the video. The legend beneath each panel corresponds to a video file.

Right Click on a number to view the video hosted on Youtube

Next: Extended Results

Navigate this article:
Borrelia Spirochete Morphology Introduction
Borrelia Spirochete Morphology Results
Borrelia Spirochete Morphology Extended Results
Borrelia Spirochete Morphology Supporting Evidence
Borrelia Spirochete Morphology Fluorescent Antibody Experiment
Borrelia Spirochete Morphology Discussion, Limitations, Conclusion and Acknowledgments
Borrelia Spirochete Morphology References

Return to Microscopy Navigation